HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA)glucose ester, and linkage of pHBA into cell walls 

 -  Bioresource Technology, 100(20), p.4836-4842, 2009 .
<br/><br/> As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet)has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA)at about 14 
<br/><br/><label>Subjects--Topical Terms: </label><br/>BETA VULGARIS<br/>METABOLIC ENGINEERING<br/>P-HYDROXYCINNAMOYL-COA HYDRATASE/LYASE<br/>P-HYDROXYBENZOIC ACID<br/>CELL WALL