TY - BOOK AU - Rahman,L. AU - Kouno,H. AU - Hashiguchi,Y. AU - Yamamoto,H. AU - Narbad,A. AU - Parr,A. AU - Walton,N. AU - Walton,N. AU - Kitamura,Y. TI - HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA)glucose ester, and linkage of pHBA into cell walls KW - BETA VULGARIS KW - METABOLIC ENGINEERING KW - P-HYDROXYCINNAMOYL-COA HYDRATASE/LYASE KW - P-HYDROXYBENZOIC ACID KW - CELL WALL N2 - As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet)has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA)at about 14 UR - https://drive.google.com/file/d/1VWf6uqnx0S_iOAelF18EVWvljIL8Ul6J/view?usp=drivesdk ER -