TY  - BOOK
AU  - De Caro,J.
AU  - Boudouard,M.
AU  - Bonicel,J.
AU  - Guidoni,A.
AU  - Desnuelle,P.
AU  - Rovery,M.
TI  - Porcine pancreatic lipase. Completion of the primary structure
KW  - LIPASE
KW  - PRIMARY STRUCTURE
KW  - PEPTIDE AGGREGATION
KW  - (PIG)
N2  - The complete primary structure of a lipase (trlacylglycerol hydrolase; EC 3.1.1.3)is presented for the first time. The porcine pancreatic enzyme which was investigated is composed of a single chain of 449 amino acids. Upon fragmentation by CNBr, five peptides were obtained. The sequence of four of them (CN I-CN IV)has already been published. The present report deals with the arrangement of the 142 amino acids of the C-terminal peptide CN V, thus completing the analysis of the whole molecule. Special problems resulting from incomplete cleavage of some peptide bonds in CN V and aggregation of large peptides were overcome using Sephadex filtration of succinylated derivatives in 50
UR  - https://drive.google.com/file/d/1kvtxi5lBA2jT5OQHiKqZBvEziyv2UIcK/view?usp=drivesdk
ER  -