TY  - BOOK
AU  - Bagi,K.
AU  - Simon,L.M.
AU  - Szajani,B.
TI  - Immobilization and characterization of porcine pancreas lipase
KW  - PORCINE PANCREAS LIPASE
KW  - IMMOBILIZATION
KW  - CROSS-LINKING
KW  - CONFORMATIONAL STABILITY
N2  - Porcine pancreas lipase (triacylglycerol ester hydrolase, EC 3.1.1.3)was immobilized with the highest activity (2,187 U g-t solid)on polyactylamide beads possessing carboxylic functional groups activated by a watersoluble carbodiimide. The optimum pH for catalytic activity was pH 8.9. The apparent optimum temperature for the immobilized enzyme was about 7°C higher than that for the soluble enzyme. The immobilization stabilized the enzyme against heat and urea treatment. Cross-linking of the immobilized enzyme with glutaraldehyde or 3,5-difluoronitrobenzene improved the thermal stability. Application of the immobilized lipase for olive oil hydrolysis is also presented
UR  - https://drive.google.com/file/d/1b6lUCJuadTMhSnkSnISy6O81QREMrik7/view?usp=drivesdk
ER  -