The use of the nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: a comparison with radioactivity 

 -  Transgenic Research, 2(2), p.115-120, 1993 .
<br/><br/> A nonradioactive labelling and detection method for plant genomic DNA analysis is compared to the radioactive method. The radioisotopes are replaced by a nucleotide, digoxigenin-11-dUTP, and the signal detection is accomplished by the enzymatic reaction of alkaline phosphatase, conjugated to anti-digoxigenin antibodies, with the chemiluminescent substrate AMPPD 3-(2'-spiroadamantane)-4-methoxy-4(3"phosphorytoxy)phenyl-1, 2-dioxetane). The sensitivity of the radioactive and nonradioactive methods are directly compared using identical Southern blots subjected to the radioactive and nonradioactive detection. The advantages of this nonradioactive method are discussed. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>CHEMILUMINESCENCE<br/>DIGOXIGENIN<br/>NONRADIOACTIVE<br/>PLANT DNA<br/>SOUTHERN BLOT HYBRIDIZATION