TY  - BOOK
AU  - Neuhaus-Url,G.
AU  - Neuhaus,G.
TI  - The use of the nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: a comparison with radioactivity
KW  - CHEMILUMINESCENCE
KW  - DIGOXIGENIN
KW  - NONRADIOACTIVE
KW  - PLANT DNA
KW  - SOUTHERN BLOT HYBRIDIZATION
N2  - A nonradioactive labelling and detection method for plant genomic DNA analysis is compared to the radioactive method. The radioisotopes are replaced by a nucleotide, digoxigenin-11-dUTP, and the signal detection is accomplished by the enzymatic reaction of alkaline phosphatase, conjugated to anti-digoxigenin antibodies, with the chemiluminescent substrate AMPPD 3-(2'-spiroadamantane)-4-methoxy-4(3"phosphorytoxy)phenyl-1, 2-dioxetane). The sensitivity of the radioactive and nonradioactive methods are directly compared using identical Southern blots subjected to the radioactive and nonradioactive detection. The advantages of this nonradioactive method are discussed
UR  - https://drive.google.com/file/d/17vGVLjlNja-QFc3bes7AbbFjy6UJzg6y/view?usp=drivesdk
ER  -