Bacterial activity and community composition in stream water and bio¢lm from an urban river determined by £uorescent in situ hybridization and DGGE analysis
- FEMS MicroBiology Ecology, 43, p.111-119, 2003 .
Physiologic activity and community structure of planktonic and biofilm microbial communities in an urban river were analyzed using 5-cyano-2,3-ditolyl tetrazolium chloride (CTC)staining, fluorescent in situ hybridization (FISH)and denaturing gradient gel electrophoresis (DGGE)analysis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments. Respiring bacteria estimated by CTC reduction were higher in biofilms (20
RIVER BIO¢LM PLANKTONIC BACTERIUM PHYSIOLOGICAL ACTIVITY MICROBIAL COMMUNITY STRUCTURE