Elastase-induced hvdrolvsis of synthetic solid subsrates: poly(ester-urea-urethane)and poly(ether-urea-urethane) 

 -  BioMaterials, 17, p.2381-2388, 1996 .
<br/><br/> Human neutrophil elastase (HNE)and porcine pancreatic elastase (PPE)were incubated with two radiolabelled model poly(urethane)s, a poly(ester-urea-urethane)containing ['4C]toluene diisocyanate ([14C]TDl), poly(caprolactone)(PCL)and ethylenediamine (ED), and a poly(ether-urea-urethane)containing [14C]TDI, poly(tetramethylene oxide)(PTMO)and ED. Ten-fold more radioactive carbon was released when PPE was incubated with ['4C]TDI/PCLIED than when HNE was used. The PPE-induced radioactive carbon release was significantly reduced by a specific elastase inhibitor. Ten-fold less radioactive carbon was released when ['4C]TDI/PTMOIED was incubated with PPE as compared to ['4C]TDI/PCL/ED. Since neutrophils, which contain elastolytic activity, are present during the inflammatory response, the stability of biomaterials used in implanted devices may be affected. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>POLY(URETHANE)S<br/>BIODEGRADATION<br/>NEUTROPHIL<br/>INFLAMMATORY RESPONSE<br/>HUMAN ELASTASE<br/>PORCINE ELASTASE