TY  - BOOK
AU  - Akio Toh-E
AU  - Shuichi Yasunagat
AU  - Hideaki Nisogit
AU  - Kazuma Tanakas
AU  - Tomoko Oguchi
AU  - Yashushi Matsui
TI  - Three Yeast Genes, PIRl, PIR2 and PIR3, Containing Internal Tandem Repeats, are Related to Each Other, and PIRI and PIR2 are Required for Tolerance to Heat Shock
KW  - GENE FAMILY
KW  - PROTEIN WITH INTERNAL REPEATS
KW  - S. CEREVISIAE: HEAT SHOCK
N2  - We isolated three highly homologous genes, PIRl, PIR2 and PIR3, collectively called the PIR genes. The remarkable feature of their putative amino acid sequence is that they contain a sequence consisting of 18-19 amino acid residues repeated tandemly seven to ten times. Genes homologous to PIR were found in Kluyveromyces lactis and Zygosaccharomyces rouxii but not in Schizosaccharomycespombe, suggesting that a set of PIR genes plays some role in budding yeast. Bias of codon usage seen in each of the PZR translation products suggests that they are expressed abundantly. The fact that disruption of each gene is viable indicates that none of them is essential. The double disruptants, pirl pir2, were viable under various conditions, such as higher temperature (37°C)or high salt concentration, but showed a slow-growing phenotype on an agar slab. Furthermore, they were sensitive to heat shock. Addition of a pir3 disruption to the pirl pir2 double disruptant brought about no phenotypic difference from the original double mutant. PIRI and PIR3 are closely link ed to each other and are on chromosome XI
UR  - https://drive.google.com/file/d/1S36-egVjVadvbyDobLV3BWz7ssF6oAOb/view?usp=drivesdk
ER  -