Axenic culture of Lemna trisulca L. 

 -  Aquatic Botany, 38(2-3), p.295-301, 1990 .
<br/><br/> Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.