TY  - BOOK
AU  - Hueberta,David B.
AU  - Mcilraitha,A.L.
AU  - Shaya,J.M.
AU  - Robinson,G.G.C.
TI  - Axenic culture of Lemna trisulca L
N2  - Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture
UR  - https://drive.google.com/file/d/16serHM7g5QQu0wXcyfzV1mvKF6uh06sJ/view?usp=drivesdk
ER  -