TY  - BOOK
AU  - Claude,B.
AU  - Viron-Lamy,C.
AU  - Haupt,K.
AU  - Morin,P.
TI  - Synthesis of a Molecularly Imprinted Polymer for the Solid-phase Extraction of Betulin and Betulinic Acid from Plane Bark
KW  - MOLECULARLY IMPRINTED POLYMER
KW  - MIP
KW  - SOLID-PHASE EXTRACTION
KW  - SPE
KW  - BETULIN
KW  - BETULINIC ACID
KW  - TRITERPENE
KW  - PLANE BARK EXTRACT
N2  - Plant extracts are usually complex mixtures of various polarity compounds and their study often includes a purifi cation step, such as solid-phase extraction (SPE), to isolate interest compounds prior analytical investigations. Molecularly imprinted polymers (MIPs)are a new promising type of SPE material which off er tailor-made selectivity for the extraction of trace active components in complex matrices. Numerous specifi c cavities that are sterically and chemically complementary of the target molecules, are formed in imprinted polymers. A molecularly imprinted polymer (MIP)was synthesised in order to trap a specifi c class of triterpene, including betulin and betulinic acid from a methanolic extract of plane bark. Methodology - Imprinted polymers were synthesised by thermal polymerisation of betulin as template, methacrylic acid (MAA)or acrylamide (AA)as functional monomer, ethylene glycol dimethacrylate as crosslinking agent and chloroform as porogen. Afterwards, MAA- and AA-MIPs were compared with their non-imprinted polymers (NIPs)in order to assess the selectivity vs betulin and its derivatives. Recovered triterpenes were analysed by HPLC during MIP-SPE protocol. Results - After SPE optimisation, the MAA-imprinted polymer exhibited highest selectivity and recovery (better than 70
UR  - https://drive.google.com/file/d/1KY-I4CuFUF3r1iRzwDr1k9SIj7GbqTlB/view?usp=drivesdk
ER  -