TY  - BOOK
AU  - Grimes,A.
AU  - Mcardle,H.J.
AU  - Mercer,J.F.B.
TI  - A Total Extract Dot Blot Hybridization Procedure for mRNA Quantitation in Small Samples of Tissues or Cultured Cells
KW  - RECOMBINANT DNA TECHNOLOGY
KW  - MRNA
KW  - RNA: DNA: GENE EXPRESSION
N2  - A simple method for the estimation of specific mRNA concentrations in small tissue samples (as little as 1 mg)or cultured cells (lower limit IO5 cells)is described. Guanidine hydrochloride extracts of whole cells or tissues are applied directly onto nitrocellulose and hybridized with the appropriate nick-translated probe. Loading according to DNA content allows expression ofthe result as concentration per cell. Hybridizing with a ribosomal RNA probe allows expression of results relative to rRNA and estimation of the RNA/DNA ratio in the sample. We describe the application of this procedure to the measurement of ceruloplasmin mRNA in tissues and cultured hepatocytes
UR  - https://drive.google.com/file/d/1gwe3XIwUi-aNhBUWf2KiNkhVlGc3pnG_/view?usp=drivesdk
ER  -