TY - BOOK AU - Vreede,F.T. AU - Cloete,M. AU - Napier,G.B. AU - Van Dijk,A.A. AU - Viljoen,G.J. TI - Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation KW - DSRNA VIRUSES KW - AFRICAN HORSE SICKNESS VIRUS (AHSV) KW - SINGLE PRIMER AMPLIFICATION N2 - A strategy was developed for sequence-independent synthesis and amplification of full-length cDNA of 3-4 kb genes of dsRNA viruses. The method of single primer amplification (Lambden et al., 1992)was adapted by the inclusion of a 3 UR - https://drive.google.com/file/d/1ACmns9xL_3rCQ2zltb6dZo6Bhh9Q9elB/view?usp=drivesdk ER -