TY  - BOOK
AU  - Ruiz-López,N.
AU  - Garcés,R.
AU  - Harwood,J.L.
AU  - Martínez-Force,E.
TI  - Characterization and partial purification of acyl-CoA:glycerol 3-phosphate acyltransferase from sunflower (Helianthus annuus L.)developing seeds
KW  - ACYLTRANSFERASE
KW  - GLYCEROL 3-PHOSPHATE
KW  - HELIANTHUS ANNUUS
KW  - MICROSOMAL MEMBRANE
KW  - SUNFLOWER
KW  - SEEDS
N2  - The glycerol 3-phosphate acyltransferase (GPAT, EC 2.3.1.15)from sunflower (Helianthus annuus L.)microsomes has been characterised and partially purified. The in vitro determination of activity was optimized, and the maximum value for GPAT activity identified between 15 and 20 days after flowering. The apparent MichaeliseMenten Km for the glycerol 3-phosphate was 354 mM. The preferred substrates were palmitoyl-CoA ¼ linoleoyl-CoA > oleoyl-CoA with the lowest activity using stearoyl-CoA. High solubilisation was achieved using 0.75
UR  - https://drive.google.com/file/d/19Yjctt8_Fgxy4-dPwM-cxQx2AG8xASuw/view?usp=drivesdk
ER  -