TY  - BOOK
AU  - Kwon,M.
AU  - Kim,H.S.
AU  - Hahm,D.
AU  - Song,J.K.
TI  - Synthesis activity-based zymography for detection of lipases and esterases
KW  - ESTERIFICATION
KW  - FATTY ACID ESTER
KW  - LIPASE
KW  - SYNTHESIS ACTIVITY
KW  - ZYMOGRAPHY
N2  - A new zymography method for lipases and esterases was developed on the basis of the esterification reaction between fatty acids and alcohols. The enzymes were separated by SDS-PAGE and native PAGE. The gel was washed and then incubated in an aqueous solution containing fatty acids (oleic acid 18:1 or caprylic acid 8:0)and dodecanol. Synthesis was visualized by in situ precipitation of water-insoluble and non-diffusible fatty acid esters, such as dodecyl oleate and dodecyl octanoate. The synthesis activity-based zymography was confirmed with different enzyme samples, including commercial lipase preparations, purified recombinant lipase and cutinase, and crude culture supernatants of lipolytic enzyme-producing soil bacteria
UR  - https://drive.google.com/file/d/1PG0KppIMp3JQ-iaBIFhAK_OWvtb-j_se/view?usp=drivesdk
ER  -