TY  - BOOK
AU  - Sussman,I.
AU  - Avron,M.
TI  - Characterization and partial purification of dl-glycerol-1-phosphatase from Dunaliella salina
KW  - DL-GLYCEROL-L-PHOSPHATASE
KW  - OSMOREGULATION
KW  - GLYCEROL METABOLISM
KW  - (DUNALIELLA SALINA)
N2  - A specific DL-glycerol-l-phosphatase (glycerol-l-phosphate phosphohydrolase, EC 3.1.3.21)has been identified in the halotolerant alga Duniella salina. The enzyme is highly specific for DL-glycerol 1-phosphate, requires magnesium for activity and has a neutral pH optimum. High sensitivity toward sulfhydryl reagents suggests the existence of a sulfhydryl group in close proximity to the active site. Due to instability the enzyme was only partially purified (40-fold). Activity measurements following polyacrylamide electrophoresis showed the enzyme to have a molecular weight around 86 kdaltons. It is suggested that the enzyme plays a major role in the mechanism of osmoregulation in Dunaliella
UR  - https://drive.google.com/file/d/1PgnZ2bQZ1hIjFMs8T_3ZB2LzFOmfcsTl/view?usp=drivesdk
ER  -