TY  - BOOK
AU  - Yamamoto,N.
AU  - Kobayashi,H.
AU  - Togashi,T.
AU  - Mori,Y.
AU  - Kikuchi,K.
AU  - Kuriyama,K.
AU  - Tokuji,Y.
TI  - Formation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor
KW  - 5-AZACYTIDINE
KW  - CARROT
KW  - DNA METHYLATION
KW  - SOMATIC EMBRYOGENESIS
N2  - Using a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also downregulated the expression of DcLEC1c, a LEC1-like embryonic gene in carrot, during morphogenesis of embryos. A carrot DNA methyltransferase gene, Met1-5 was expressed transiently after the induction of somatic embryogenesis by 2,4- dichlorophenoxyacetic acid (2,4-D), before the formation of embryogenic cell clumps. These findings suggested the significance of DNA methylation in acquiring the embryogenic competence in somatic cells in carrot
UR  - https://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk
ER  -