TY  - BOOK
AU  - Nishida,I.
AU  - Sugiura,M.
AU  - Enju,A.
AU  - Nakamura,M.
TI  - A Second Gene for Acyl-(Acyl-Carrier-Protein): Glycerol-3-Phosphate Acyltransferase in Squash, Cucurbita moschata cv. Shirogikuza*, Codes for an Oleate-Selective Isozyme: Molecular Cloning and Protein Purification Studies
N2  - A new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15)in squash has been cloned and the gene product was identified as oleate- selective GPAT. Using PCR primers that could hybridise with exons for a previously cloned squash GPAT, we obtained two PCR products of different size: one coded for a previously cloned squash GPAT corresponding to non-selective isoforms AT2 and AT3, and the other for a new isozyme, probably the oleate-selective isoform AT1. Fulllength amino acid sequences of respective isozymes were deduced from the nucleotide sequences of genomic genes and cDNAs, which were cloned by a series of PCR-based methods. Thus, we designated the new gene CmATS1;1 and the other one CmATS1;2. Genome blot analysis revealed that the squash genome contained the two isogenes at nonallelic loci. AT1-active fractions were partially purified, and three polypeptide bands were identified as being AT1 polypeptides, which exhibited relative molecular masses of 39.5-40.5 kDa, pI values of 6.75-7.15, and oleate selectivity over palmitate. Partial amino-terminal sequences obtained from two of these bands verified that the new isogene codes for AT1 polypeptides
UR  - https://drive.google.com/file/d/1h2X_GiqDrIQQhj2ro7qQVs911GIrWLnn/view?usp=drive_link
ER  -