TY  - BOOK
AU  - Ruiz-Herrera,J.
AU  - Ruiz-Medrano,R.
AU  - Dominguez,A.
TI  - Selective inhibition of cytosine-DNA methylases by polyamines
KW  - PUTRESCINE
KW  - SPERMIDINE
KW  - DNA-METHYLATION
KW  - RESTRICTION ENZYME
N2  - We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine- DNA methyltranferases is rather selective and suggest a possible mode of action in vivo
UR  - https://drive.google.com/file/d/1Nw1MdMkeeIWcT168g2nIYrsWoEVuCZ9K/view?usp=drivesdk
ER  -