TY  - BOOK
AU  - Syed Salman Lateef
AU  - Shalini Gupta
AU  - Lasanthi P.Jayathilaka
AU  - Sangeeth Krishnanchettiar
AU  - Jin-Sheng Huang
AU  - Bao-Shiang Lee
TI  - An Improved Protocol for Coupling Synthetic Peptides to Carrier Proteins for Antibody Production Using DMF to Solubilize Peptides
KW  - PEPTIDE
KW  - ANTIBODY
KW  - KLH
KW  - BSA
KW  - MBS
KW  - DMF
KW  - MALDI- TOF MS
N2  - We present an improved protocol for coupling synthetic peptides to carrier proteins. In this protocol, dimethylformamide is used as the solvent to solubilize peptides instead of phosphate-buffered saline (PBS)or 6 M guanidine-HC l/0.01 M phosphate buffer (pH 7). Additionally, the last desalting or dialyzing step to remove uncoupled peptides as in the traditional method is eliminated. Finally, 3 mL of 0.1 M ammonium bicarbonate is added to the carrier protein conjugated peptide solution to help the lyophilization process. Coupling of Cys-containing synthetic peptides to keyhole limpet hemocyanin or bovine serum albumin using mmaleimidobenzoyl- N-hydroxysuccinimide ester are used as the test cases. This method produces high-quality antipeptide antibodies. Also, compared to the traditional method, this procedure is simpler and useful for peptides with solubility problems in PBS or 6 M guanidine-HC l
UR  - https://drive.google.com/file/d/1qQBDR1MayE9oarTAoX-ADGzzSYtE4b2l/view?usp=drivesdk
ER  -