TY  - BOOK
AU  - Turgeon,B.G.
AU  - Condon,B.
AU  - Liu,J.
AU  - Zhang,N.
TI  - Protoplast Transformation of Filamentous Fungi
KW  - SELECTABLE MARKER
KW  - DRUG RESISTANCE
KW  - SPLIT-MARKER
KW  - GENE-DELETION
KW  - COTRANSFORMATION
KW  - HYGROMYCIN B
KW  - GENETICIN
KW  - BIALAPHOS
KW  - PHOSPHOTHRICIN
KW  - REMI
KW  - INTEGRATION
N2  - The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI)procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed
UR  - https://drive.google.com/file/d/10XWa9Prehse4f3jSjl1EeaELw7Cr_uq0/view?usp=drivesdk
ER  -