High Performance Liquid Chromatographic Determination of Subpicomole Amounts of Amino Acids by Precolumn Fluorescence Derivatization with o-Phthaldialdehyde
- Analytical Chemistry, 51, p.1667-1674, 1979 .
Using reversed-phase high performance llquM chromatography, 25 amino acids as o-phthaidialdehyde derivatives were determined with a 30-min sample turnover. The derivatization procedure is rapid, performed in aqueous medium, and has few transfer steps. The precision is *OS