Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells 

 -  CryoLetters, 29(5), p.427-436, 2008 .
<br/><br/> We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g H2O g DW-1)followed by immersed in LN. After rewarming at 35°C for 3 min and 1-d incubation under continuous illumination at 22°C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ARABIDOPSIS<br/>CRYOPRESERVATION<br/>ENCAPSULATION-DEHYDRATION<br/>SUSPENSION-CULTURED CELLS<br/>METABOLIC PROFILING<br/>GC-TOF-MS