TY  - BOOK
AU  - Ogawa,Y.
AU  - Suzuki,H.
AU  - Sakurai,N.
AU  - Aoki,K.
AU  - Saito,K.
AU  - Shibata,D.
TI  - Cryopreservation and metabolic profiling analysis of Arabidopsis T87 suspension-cultured cells
KW  - ARABIDOPSIS
KW  - CRYOPRESERVATION
KW  - ENCAPSULATION-DEHYDRATION
KW  - SUSPENSION-CULTURED CELLS
KW  - METABOLIC PROFILING
KW  - GC-TOF-MS
N2  - We established a simple cryopreservation protocol for Arabidopsis T87 cells using an encapsulation-dehydration method. T87 cells were encapsulated into alginate beads containing 2 M glycerol and 0.4 M sucrose. Alginate beads containing T87 cells were dehydrated with silica gel for 2 h (to c. 0.7 g H2O g DW-1)followed by immersed in LN. After rewarming at 35°C for 3 min and 1-d incubation under continuous illumination at 22°C, cryopreserved T87 cells exhibited considerable regrowth. Exponentially-grown 7-d-old T87 cells regrew more vigorously (86
UR  - https://drive.google.com/file/d/1vnN9yApo8K5_0csja9RQLU2Y6R1rH72I/view?usp=drivesdk
ER  -