TY - BOOK AU - Kamada,H. AU - Harada,H. TI - Studies on nitrogen metabolism during somatic embryogenesis in carrot. I. Utilization of α-alanine as a nitrogen source KW - A-ALANINE KW - ALANINE AMINOTRANSFERASE KW - NITROGEN SOURCE KW - SOMATIC EMBRYOGENESIS KW - DAUCUS CAROTA L N2 - a-Alanine added to a culture medium was incorporated into cells and/or embryos during rapid cell proliferation and globular embryo formation, in which an active protein synthesis was occurring. After the incorporation into cells, a-alanine seemed to be quickly transformed to glutamic acid by alanine aminotransferase and utilized as a nitrogen source. Alanine aminotransferase activity was observed in cells and/or embryos, although the activity decreased during culture period. Utilization of a-alanine by cultured cells as a nitrogen source was discussed in relation to its stimulative effect on somatic embryogenesis UR - https://drive.google.com/file/d/1q8J-iRo42nF6Se2rB_QQQ10GYsPuDJ6R/view?usp=drivesdk ER -