An On-Line HPLC Method for Detection of Radical Scavenging Compounds in Complex Mixtures 

 -  Anal. Chem., 72(10), p.2323-2328, 2000 .
<br/><br/> A rapid on-line method for screening of complex mixtures for radical scavenging components was developed using a methanolic solution of 2,2¢-diphenyl-1-picrylhydrazyl (DPPH)stable free radical. The HPLC-separated analytes react postcolumn with the DPPH solution, and the induced bleaching is detected as a negative peak by an absorbance detector at 517 nm. An optimized instrumental setup is presented. The method is suitable for both isocratic and gradient HPLC runs with mobile-phase compositions ranging from 10 to 90