TY - BOOK AU - Koleva,I.I. AU - Niederlander,H.A.G. AU - Van Beek,T.A. TI - An On-Line HPLC Method for Detection of Radical Scavenging Compounds in Complex Mixtures N2 - A rapid on-line method for screening of complex mixtures for radical scavenging components was developed using a methanolic solution of 2,2ยข-diphenyl-1-picrylhydrazyl (DPPH)stable free radical. The HPLC-separated analytes react postcolumn with the DPPH solution, and the induced bleaching is detected as a negative peak by an absorbance detector at 517 nm. An optimized instrumental setup is presented. The method is suitable for both isocratic and gradient HPLC runs with mobile-phase compositions ranging from 10 to 90 UR - https://drive.google.com/file/d/17ruTzrhTDMf6h41626K9kaGr7nHVtFYo/view?usp=drivesdk ER -