TY  - BOOK
AU  - Chang,Y.
AU  - Chuang,H.
AU  - Meksem,K.
AU  - Wu,F.
AU  - Chang,C.
AU  - Zhang,M.
AU  - Zhang,H.
TI  - Characterization of a plant-transformation-ready large-insert BIBAC library of Arabidopsis and bombardment transformation of a large-insert BIBAC of the library into tobacco
KW  - ARABIDOPSIS THALIANA
KW  - HIGH-MOLECULAR-MASS DNA TRANSFORMATION
KW  - BINARY BACTERIAL ARTIFICIAL CHROMOSOME
KW  - FUNCTIONAL GENOMICS
KW  - MOLECULAR BREEDING
N2  - Plant-transformation-ready, large-insert binary bacterial artificial chromosome (BIBAC)libraries are of significance for functional and network analysis of large genomic regions, gene clusters, large-spanning genes, and complex loci in the postgenome era. Here, we report the characterization of a plant-transformation-ready BIBAC library of the sequenced Arabidopsis g nome for which such a library is not available to the public, the transformation of a large-insert BIBAC of the library into tobacco by biolistic bombardment, and the expression analysis of its containing genes in transgenic plants. The BIBAC library was constructed from nuclear DNA partially digested with BamHI in the BIBAC vector pCLD04541. It contains 6144 clones and has a mean insert size of 108 kb, representing 5.2× equivalents of the Arabidopsis genome or a probability of greater than 99
UR  - https://drive.google.com/file/d/1-YbjRGie_7FZDFempztfucwY53fj0fhQ/view?usp=drivesdk
ER  -