TY  - BOOK
AU  - Wang,T.
AU  - Ma,X.
AU  - Zhu,H.
AU  - Li,A.
AU  - Du,G.
AU  - Chen,J.
TI  - Available methods for assembling expression cassettes for synthetic biology
KW  - SYNTHETIC BIOLOGY
KW  - SIMULTANEOUS EXPRESSION
KW  - PATHWAY CONSTRUCTION
KW  - YEAST RECOMBINATION
KW  - CRE-LOXP SITESPECIFIC
KW  - RECOMBINATION
KW  - ACEMBL SYSTEM
N2  - Studies in the structural biology of the multicomponent protein complex, metabolic engineering, and synthetic biology frequently rely on the efficient over-expression of these subunits or enzymes in the same cell. As a first step, constructing the multiple expression cassettes will be a complicated and time-consuming job if the classic and onventional digestion and ligation based cloning method is used. Some more efficient methods have been developed, including (1)the employment of a multiple compatible plasmid expression system, (2)the rare-cutter-based design of vectors, (3)in vitro recombination (sequence and ligation independent cloning, the isothermally enzymatic assembly of DNA molecules in a single reaction), and (4)in vivo recombination using recombination-efficient yeast (in vivo assembly of overlapping fragments, reiterative recombination for the chromosome integration of foreign expression cassettes). In this review, we systematically introduce these available methods
UR  - https://drive.google.com/file/d/1iDipvGZwCXAnfZSq2I7vtolbEgKTddh_/view?usp=drivesdk
ER  -