TY - BOOK AU - Frey,U.H. AU - Bachmann,H.S. AU - Peters,J. AU - Siffert,W. TI - PCR-amplification of GC-rich regions: 'slowdown PCR' N2 - The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483 UR - https://drive.google.com/file/d/1sRGI3fOEhk7PMmmc5kvZwguaoskiX4Mk/view?usp=drivesdk ER -