Isolation of acetylcholine receptors by chloroform-methanol extraction: Artifacts arising in use of Sephadex LH-20 columns
- Biochimica et Biophysica Acta, 288(1), p.241-247, 1972 .
Attempts were made to isolate acetylcholine receptors from the electric organ of Electrophorus electricus by means of chloroform-methanol extraction and subsequent purification on Sephadex LH-20 columns. Evidence is presented which indicates that the "receptor"-ligand binding observed may be artifactual.