IN VITRO CULTURE OF ALOE BARBADENSIS Mill.: MORPHOGENETIC ABILITY AND NUCLEAR DNA CONTENT 

 -  Plant Science, 55(1), p.53-59, 1988 .
<br/><br/> In vitro culture of Aloe barbadensis is very difficult for both callus induction and plant regeneration. By contrast, micropropagation from shoot meristems is easily achieved. A DNA microdensitometric study was performed on different organs of A. barbadensis and during in vitro culture of different explants. It was shown that morphogenetic ability is correlated to nuclear DNA content in the explant and/or in the callus. In particular, plant regeneration (or micropropagation)is obtained only from caUi (or from explants)in which the amount of DNA per nucleus falls strictly within the diploid limits (2C and 4C)of the species. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ALOE BARBADENSIS<br/>CALLUS<br/>CHROMOSOME ENDOREDUPLICATION<br/>CYTOPHOTOMETRY<br/>NUCLEAR DNA CONTENT<br/>PLANT REGENERATION