TY  - BOOK
AU  - Tam,Y.Y.
AU  - Normanly,J.
TI  - Determination of indole-3-pyruvic acid levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry
KW  - ARABIDOPSIS THALIANA
KW  - DERIVATIZATION, GC
KW  - INDOLEPYRUVIC ACID
KW  - PENTAFLUOROPHENYLHYDRAZINE
KW  - PENTAFLUOROBENZYLHYDROXYLAMINE
N2  - A rapid and simple method is described for the determination of indole-3-pyruvic acid (IPA)levels in Arabidopsis thaliana by gas chromatography-selected ion monitoring-mass spectrometry (GC-SIM-MS). The method includes derivatization of IPA with hydroxylamine in the crude extract, followed by ethyl acetate partitioning, solid-phase extraction with C resin, reversed-phase high-performance liquid chromatography (HPLC), and GC-SIM-MS. Three derivatizing 18 reagents were tested; these were entafluorobenzylhydroxylamine, pentafluorophenylhydrazine, and hydroxylamine. Hydroxylamine proved to be the most useful, as the IPA-oxime was easiest to purify from plant extracts and was the most stable. IPA was quantified in Arabidopsis seedlings ranging in age from 5 to 12 days; levels varied from 4 to 13 ng/ g, peaking at 7-9 days
UR  - https://drive.google.com/file/d/1P0PNyPuM9oprfio3F9dBIN_BNzb2hKd3/view?usp=drivesdk
ER  -