TY  - BOOK
AU  - Ermolaeva,O.D.
AU  - Sverdlov,E.D.
TI  - Subtractive hybridization, a technique for extraction of DNA sequences distinguishing two closely related genomes: critical analysis
KW  - SUBTRACTIVE HYBRIDIZATION
KW  - ENRICHMENT
KW  - KINETICS
N2  - The present status of genomic DNA subtraction techniques is reviewed. The advantages and disadvantages of the widely-used methods of genome subtrac.tion are discussed. Using the kinetic model of subtractive hybridization developed by us previously (Sverdlov and Ermolaeva, ]993; Sverdlov and Ermolaeva, 1994), the application of genome subtraction to various problems is analyzed. It is concluded that the technique should be further advanced based on subtraction of single-stranded DNAs. This strategy would enable one to efficiently extract target sequences omitting the stage of genome simplification
UR  - https://drive.google.com/file/d/1BDeec3jdMNSyZ5OXU_tTk-O1zdhvT6fD/view?usp=drivesdk
ER  -