TY  - BOOK
AU  - Zhang,Yongjiang
AU  - Zhao,Wenjun
AU  - Li,Mingfu
AU  - Chen,Hongjun
AU  - Zhu,Shuifang
TI  - Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds
KW  - MAIZE CHLOROTIC MOTTLE VIRUS
KW  - REAL-TIME RT-PCR
KW  - QUARANTINE VIRUS
N2  - Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing
UR  - https://drive.google.com/file/d/1Hks_oBYSQEIv2TZW_DCAGdQLPxd16zdu/view?usp=drivesdk
ER  -