TY  - BOOK
AU  - Shvetsov,S.G.
AU  - Gamburg,K.Z.
TI  - Uptake of 2,4-Dichlorophenoxyacetic Acid by Suspension-cultured Maize Cells
N2  - The uptake of 2,4-D-[1-14C]by maize cells was studied in relation to 2,4-D concentration, density of suspension, growth phase of subculture and pH of the medium. The uptake was estimated by the equilibrium 2,4-D content in the cells (Ot)and by the relative rate of the approach of cell 2,4-D content to Ot (k)which was shown to be constant during the uptake. An increase in suspension density caused a decrease in Ot and an enhancement of le. The exponential phase cells (4th day of subculture)took up 2,4-D more actively than the stationary phase cells just transferred to the fresh medium. This activation of uptake can only partly be conditioned by the acidification of the medium during the subculture. An increase in 2,4-D concentration in the medium from 1 mg!l to 5-10 mgjl caused an enhancement of k in the initial culture and its decrease in the 4th day culture. Only part of absorbed 2,4-D could be washed out by three successive 1 h incubations of the cells with the medium without 2,4-D. The non-removed (or very slowly removed)2,4-D was equal near to 70 percent of total absorbed 2,4-D in the cells of the 4th day culture and 20 percent in the cells of the initial culture
UR  - https://drive.google.com/file/d/1BMKEss6C1qZgWedBvfodBHyMe-n49E_G/view?usp=drivesdk
ER  -