A rapid response to a plant hormone: auxin stimulates phospholipase a, in vivo and in vitro
- BioChemical and Biophysical Research Communications, 163(1), p.111-117, 1989 .
Addition of the active auxins indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid or a-naphthylacetic acid to cultured soybean (Glycine max L.)cells prelabeled with ethanolamine or choline increased the radioactivity in the lysophosphatidylethanolamine (LPE)or lysophosphatidylcholine (LPC)pool within 5 min. The inactive auxin analogue, P-naphthylacetic acid, was inactive in this response. In membranes prelabeled in vivo, either with ethanolamine or choline, and subsequently isolated from zucchini (Cucurbita pepo L.)hypocotyls, indole- 3-acetic acid and 2,4-dichlorophenoxyacetic acid stimulated the conversion of phosphatidylethanolamine (PE)to LPE and of phosphatidylcholine (PC)to LPC in vitro whereas the inactive auxin analogue 2,3-dichlorophenoxyacetic acid did not.