TY  - BOOK
AU  - Huitema,E.
AU  - Smoker,M.
AU  - Kamoun,S.
TI  - A Straightforward Protocol for Electro-transformation of Phytophthora capsiciZoospores
KW  - OOMYCETES
KW  - PHYTOPHTHORA CAPSICI
KW  - GENETIC TRANSFORMATION
KW  - ZOOSPORE
KW  - ELECTROPORATION
N2  - Genome sequencing combined with high-throughput functional analyses has proved vital in our quest to understand oomycete-plant interactions. With the identification of effector molecules from Phytophthora spp. we can now embark on dissecting the mechanisms by which effectors modulate host processes and thus ensure parasite fitness. One of the key limitations, however, is to genetically modify Phytophthora and assess gene function during parasitism. Here, we describe a straightforward protocol that allows rapid transformation of Phytophthora capsici, an emerging model in oomycete biology. P. capsiciis a broad host range pathogen that can infect a wide variety of plants under lab conditions making it a suitable model for detailed studies on oomycete-host interactions. This protocol relies on electroporation-assisted uptake of DNA in to motile zoospores and allows the rapid identification and characterization of geneti-cally stable transformants
UR  - https://drive.google.com/file/d/1_8XHxMmrOj9rGQ7bwsmQFAHWRbHKVrln/view?usp=drivesdk
ER  -