Three Methods for the Introduction of Foreign DNA intoAgrobacterium 

 -  Methods in Molecular Biology, 343, p.43-53, 2009 .
<br/><br/> The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ELECTROPORATION<br/>FREEZE/THAW<br/>TRIPARENTAL MATING<br/>CONJUGATION<br/>INCOM-PATIBILITY GROUP<br/>SELECTIVE PLATES<br/>COUNTERSELECTION<br/>DONOR<br/>RECIPIENT<br/>TRANSCONJUGANT<br/>SELF-TRANSMISSIBLE PLASMID<br/>ORIGIN OF TRANSFER (ORIT).