TY  - BOOK
AU  - Wise,A.A.
AU  - Liu,Z.
AU  - Binns,A.N.
TI  - Three Methods for the Introduction of Foreign DNA intoAgrobacterium
KW  - ELECTROPORATION
KW  - FREEZE/THAW
KW  - TRIPARENTAL MATING
KW  - CONJUGATION
KW  - INCOM-PATIBILITY GROUP
KW  - SELECTIVE PLATES
KW  - COUNTERSELECTION
KW  - DONOR
KW  - RECIPIENT
KW  - TRANSCONJUGANT
KW  - SELF-TRANSMISSIBLE PLASMID
KW  - ORIGIN OF TRANSFER (ORIT)
N2  - The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating
UR  - https://drive.google.com/file/d/19roaQ8d2vcliIUkWpFdlISlqf3bhTh0A/view?usp=drivesdk
ER  -