Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana : Method 3
- Methods in Molecular Biology, 990, p.109-118, 2013 .
Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana . This chapter describes fi xation, sample preparation, and immunolocalization techniques used in our laboratory. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation.