TY - BOOK AU - Sisson,T.H. AU - Castor,C.W. TI - An improved method for immobilizing IgG antibodies on protein A-agarose KW - IGG KW - PROTEIN A N2 - This report describes a modification of a procedure developed by others for crosslinking IgG to protein A which itself is covalently linked to a gel support. Earlier immunoaffinity columns were described as having large antigen-binding capacities and stability under a variety of elution conditions. The present data show that columns constructed with earlier techniques were only partially stable to pH 3.0 buffers, and, as a result, bound less than 20 UR - https://drive.google.com/file/d/1YGlortSr7Zfc9Li41nRABPpiNC3RmInn/view?usp=drivesdk ER -