TY  - BOOK
AU  - Sahl,J.W.
AU  - Fairfield,N.
AU  - Harris,J.K.
AU  - Wettergreen,D.
AU  - Stone,W.V.
AU  - Spear,J.R.
TI  - Novel Microbial Diversity Retrieved by Autonomous Robotic Exploration of the World's Deepest Vertical Phreatic Sinkhole
KW  - AUTONOMOUS
KW  - ROBOTICS
KW  - MICROBIAL DIVERSITY
KW  - 16S RRNA. ASTROBIOLOGY
N2  - The deep phreatic thermal explorer (DEPTHX)is an autonomous underwater vehicle designed to navigate an unexplored environment, generate high-resolution three-dimensional (3-D)maps, collect biological samples based on an autonomous sampling decision, and return to its origin. In the spring of 2007, DEPTHX was deployed in Zacato´n, a deep (*318m), limestone, phreatic sinkhole (cenote)in northeastern Mexico. As DEPTHX descended, it generated a 3-D map based on the processing of range data from54 onboard sonars. The vehicle collected water column samples and wall biomat samples throughout the depth profile of the cenote. Post-expedition sample analysis via comparative analysis of 16S rRNA gene sequences revealed a wealth of microbial diversity. Tradi-tional Sanger gene sequencing combined with a barcoded-amplicon pyrosequencing approach revealed novel, phylum-level lineages from the domains Bacteria and Archaea; in addition, several novel subphylum lineages were also identified. Overall, DEPTHX successfully navigated and mapped Zacato´n, and collected biological samples based on an autonomous decision, which revealed novel microbial diversity in a previously unexplored environment
UR  - https://drive.google.com/file/d/1p6zAzYfw5MMNIsGzxENV571m7mJajHBP/view?usp=drivesdk
ER  -