DNA Isolation from Mammalian Samples 

 -  Current Protocols in Molecular Biology, 102(1), p.2-14, 2013 .
<br/><br/> DNA isolation is a fundamental initial step for molecular genetic studies. Two quite dif-ferent methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE)tissue samples. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>DNA ISOLATION<br/>SILICA SPIN COLUMN<br/>FFPE (FORMALIN-FIXED PARAFFIN-EMBEDDED)TISSUE<br/>AUTOMATED EXTRACTION