TY  - BOOK
AU  - Gao,W.
TI  - Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis
KW  - TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS
KW  - TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
KW  - PROTEIN SEPARATION
KW  - PROTEOMICS
N2  - A protocol for protein analysis using two-dimensional difference gel electrophoresis (2D-DIGE)is described. 2D-DIGE is one of the most popular and versatile methods of protein separation among rapidly increasing proteomics technologies. Similar to traditional two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the proteins are separated based on their charges and molecular weight by 2D-DIGE. Different from 2D-PAGE, proteins are pre-labeled with different fl uorescent and different protein samples are run in one gel by this method. Therefore, 2D-DIGE not only carries the advantages of 2D-PAGE but also eliminates gel-to-gel variation and achieves high resolution, sensitivity, and reproducibility
UR  - https://drive.google.com/file/d/1U6IQUr5SeaUBbagwiD4bqFQJsSFBQ_ho/view?usp=drivesdk
ER  -