TY  - BOOK
AU  - Wagner,W.
AU  - Keller,F.
AU  - Wiemken,A.
TI  - Fructan matabolism in cereals: induction in leaves and compartmentation in protoplasts and vacuoles
KW  - HORDEUM VULGARE
KW  - TRITICUM AESTIVUM
KW  - ASSIMILATE PARTITIONING
KW  - COLD STRESS
KW  - FRUCTAN
KW  - PROTOPLASTS
KW  - VACUOLES
N2  - Leaf blades of Triticum aestivum L. cv Kolibri and Hordeum vulgare L. cv Gerbel were induced to accumulate fructan (polyfructosylsucrose of varying molecular size)in large amounts (~70 percent of dwt)by impeding the export of photosynthates. This was achieved by sub­ jecting plants to cold stress (5°C at night)or by continuous illumination of excised leaf blades. Concomitantly with the accumulation of fructan in the leaves the activity of a sucrose­ sucrose-fructosyltransferase (SST), probably the key-enzyme of fructan anabolism, increased several fold in cell-free extracts. Its pH optimum is 5.7. At 8°C its activity is still half of the activity at 28°C, the temperature optimum. This remarkably anomalous dependence on tem­perature is interesting with regard to fructan accumulation in grasses during the cold season. Protoplasts obtained from fructan-enriched barley leaves were employed for the isolation of vacuoles. All the fructans (~trisaccharide)as well as the SST activity were found to be asso­ ciated exclusively with the vacuoles, which therefore appear to play the central role in fructan storage and metabolism
UR  - https://drive.google.com/file/d/1-aU-vu9zTPa1Ulv1NC15tjiwfaibR90q/view?usp=drivesdk
ER  -