TY  - BOOK
AU  - Arcioni,S.
AU  - Davey,M.R.
AU  - Dos Santos,A.V.P.
AU  - Cocking,E.C.
TI  - Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa
KW  - MEDICAGO COERULEA
KW  - M GLUTINOSA
KW  - BAP
KW  - CELL SUSPENSION PROTOPLASTS
KW  - MESOPHYLL PROTOPLASTS
KW  - DIVISION
KW  - 2,4·D
KW  - FORAGE LEGUMES
KW  - GENETIC MANIPULATION
KW  - SOMATIC EMBRYOGENESIS
KW  - ZEATIN
N2  - Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed
UR  - https://drive.google.com/file/d/17ZMoqWWZU0fW8IHL59tpreVejysmvb2n/view?usp=drivesdk
ER  -