Asexual Embryogenesis via Callus of Theobroma cacao 1 

 -  Z. Pflanzenphysiol., 13, p.347-358., 1984 .
<br/><br/> Callus of Theobroma cacao L. (cacao)possessing embryogenic competence occurred spontaneously with two clones of asexual embryos proliferated in vitro in a hormone-free basal medium by hypocotylary budding. Asexual embryogenesis via callus occurred at low frequency in the hormone-free basal medium. High concentrations of 2,4-D plus coconut water stimulated callus production and suppressed embryo production. Maximum frequency and intensity of embryogenesis occurred at 10-3 to 10-2 mg ·12,4-D. Embryos originated from meristematic tissue at the periphery of callus clumps. During development asexual embryos either remained embedded in the callus or were connected through suspensor-like structures. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>THEOBROMA CACAO<br/>ASEXUAL EMBRYOGENESIS<br/>CALLUS