TY - BOOK AU - Kononowicz,H. AU - Kononowicz,A.K. AU - Janick,J. TI - Asexual Embryogenesis via Callus of Theobroma cacao 1 KW - THEOBROMA CACAO KW - ASEXUAL EMBRYOGENESIS KW - CALLUS N2 - Callus of Theobroma cacao L. (cacao)possessing embryogenic competence occurred spontaneously with two clones of asexual embryos proliferated in vitro in a hormone-free basal medium by hypocotylary budding. Asexual embryogenesis via callus occurred at low frequency in the hormone-free basal medium. High concentrations of 2,4-D plus coconut water stimulated callus production and suppressed embryo production. Maximum frequency and intensity of embryogenesis occurred at 10-3 to 10-2 mg ยท12,4-D. Embryos originated from meristematic tissue at the periphery of callus clumps. During development asexual embryos either remained embedded in the callus or were connected through suspensor-like structures UR - https://drive.google.com/file/d/1f0CDxpHyWd0jjNQa2yOPuRkk7u6Niqfu/view?usp=drivesdk ER -